In the following videos, the group of IESL-FORTH presents the research activities related to FAST-DOT project:

Video 1 shows an animated time-lapse sequence in a 3-D reconstruction of Third Harmonic Generation (THG) signals arising from Caenorhabditis elegans (C. elegans) embryos. Five optical sections (2μm apart) are used for the reconstruction. The whole procedure of data acquisition needed for the 3-D reconstruction of THG images lasted 5 seconds. The sample was imaged for 25 minutes and the time-lapse between 3-D images is 2 minutes. THG signal is depicted in cyan.

Video 2 presents 2-D THG signals from a C.  elegans embryo at different axial (z) depths. Ten optical sections (2μm apart) are employed. The resolution of each image is 500x500 points. THG signal is depicted in cyan.

Video 3 depicts a rotation of a 3-D reconstruction of THG signals arising from of an early-stage C. elegans embryo. Ten optical sections (2μm apart) are used for the reconstruction. The resolution of each image is 700x700 points. THG signal is depicted in green.