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Highlights and Background

FAST-DOT is an experimental program funded under the Seventh Framework Programme of the European Union, (Photonic Components and subsystems).
Beginning in June 2008 and running for 2 years, FAST-DOT purpose is to:

- Enable widespread application and further development of laser based photonics

- Demonstrate new applications of lasers in biotechnology and medical fields

- Develop new industrially integrated design rules for the production of specific QD materials

- Unlock the potential of QD materials in biophotonics

- Accelerate the implementation of QD lasers through European SMEs and companies

- Train a new generation of researchers in the range of new technological areas for QD devices.

 

Fast Dot Project will...


- Exploit the unique combination of ultrafast properties and key wavelengths available from quantum-dot (QD) materials to produce a new generation of compact ultrafast laser devices

- Engineer specific novel properties available due to control of the growth of QD

- Implement a new range of ultrafast QD lasers for important biophotonics and medical applications.

 



Two-photon imaging

Two-photon imaging demonstration



 
Two photon imaging and 3D reconstruction of a GFP expressing C. Elegans nerve cell ring
 
 

 Two photon imaging and 3D Reconstruction of a GFP labled mouse kidney tissue sample

 

 

 

 

 

 

 

 

 

 

 

 

 

                    Before axon cutting                                                                                 After axon cutting

Axotomie C.Elegans with simultaneous two photon imaging.

 
Demonstration of third harmonic generation (THG)

In the following videos, the group of IESL-FORTH presents the research activities related to FAST-DOT project:

 

Video 1 shows an animated time-lapse sequence in a 3-D reconstruction of Third Harmonic Generation (THG) signals arising from Caenorhabditis elegans (C. elegans) embryos. Five optical sections (2μm apart) are used for the reconstruction. The whole procedure of data acquisition needed for the 3-D reconstruction of THG images lasted 5 seconds. The sample was imaged for 25 minutes and the time-lapse between 3-D images is 2 minutes. THG signal is depicted in cyan.

 

 

 

 

Video 2 presents 2-D THG signals from a C.  elegans embryo at different axial (z) depths. Ten optical sections (2μm apart) are employed. The resolution of each image is 500x500 points. THG signal is depicted in cyan.

 

 

 

 

Video 3 depicts a rotation of a 3-D reconstruction of THG signals arising from of an early-stage C. elegans embryo. Ten optical sections (2μm apart) are used for the reconstruction. The resolution of each image is 700x700 points. THG signal is depicted in green.

 
WP5 highlights


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